Coding

Part:BBa_K5236012:Design

Designed by: Allen Yang   Group: iGEM24_BASIS-China   (2024-09-30)


BhrPETase M57L


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 226
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For expressing the sequence, signal peptides and lac operator are suggested to consider when designing a pathway.


Source

Mutation site predicted by AI

References